Figure 3. IL-21 is primarily produced by... | Rockefeller University Press

Figure 3.

$Figure 3. IL-21 is primarily produced by brain-infiltrating CD4+ T cells. (a) Intracellular cytokine staining of lymphocytes isolated from n = 5 pooled healthy WT, ischemic IL-21−/−, or ischemic WT mouse brains 24 h after tMCAO or sham procedure showing IL-21 versus CD8α expression. Histograms show CD4, NK1.1, and TCRγδ expression on IL-21+ cells from ischemic WT brain. (b) CD45, CD4, and LFA-1 expression by negative fractions purified from WT and IL-21−/− lymph node cells by CD4+ negative selection using magnetic cell separation before transfer into RAG2−/− recipients. (c) Infarct volume in WT mice (n = 4), RAG2−/− mice (n = 4), RAG2−/− mice + WT CD4 T cells (n = 10), and RAG2−/− mice + IL-21−/− CD4 T cells (n = 10) 24 h after tMCAO. Representative TTC-stained 2-mm mouse brain slices shown on top. Data are representative of two independent experiments. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 one-way ANOVA. Error bars indicate SD.$

IL-21 is primarily produced by brain-infiltrating CD4⁺ T cells. (a) Intracellular cytokine staining of lymphocytes isolated from n = 5 pooled healthy WT, ischemic IL-21^−/−, or ischemic WT mouse brains 24 h after tMCAO or sham procedure showing IL-21 versus CD8α expression. Histograms show CD4, NK1.1, and TCRγδ expression on IL-21⁺ cells from ischemic WT brain. (b) CD45, CD4, and LFA-1 expression by negative fractions purified from WT and IL-21^−/− lymph node cells by CD4⁺ negative selection using magnetic cell separation before transfer into RAG2^−/− recipients. (c) Infarct volume in WT mice (n = 4), RAG2^−/− mice (n = 4), RAG2^−/− mice + WT CD4 T cells (n = 10), and RAG2^−/− mice + IL-21^−/− CD4 T cells (n = 10) 24 h after tMCAO. Representative TTC-stained 2-mm mouse brain slices shown on top. Data are representative of two independent experiments. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 one-way ANOVA. Error bars indicate SD.

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