Figure 2.
Figure 2. The 241-242TC Il7r vector induces ETP-ALL with a myeloid phenotype when introduced into immature thymocytes. (A) A peripheral blood smear from leukemia induced by the Il7r 241-242TC mutant in a recipient mouse 64 d after transplant. Bar, 10 µm. (B) Co-expression of the vector (GFP) and the myeloid lineage marker Gr1 in leukemic cells from the spleen, BM, and peripheral blood as analyzed by flow cytometry. (C) Leukemic infiltrates were present in the kidney and other solid organs and tumor cells expressed myeloperoxidase (MPO) and GFP by immunohistochemistry. Bars, 75 µm. (D) PCR analysis for TCR rearrangements in ETP-ALL tumor cells. Two Il7r 241-242TC mutant ETP-ALL leukemic samples from the spleen (SP1, SP2) have monoclonal TCR rearrangements. Other controls show the unrearranged germline TCR in BM granulocytes and polyclonal TCR rearrangements (multiple bands) in normal DN thymocytes.

The 241-242TC Il7r vector induces ETP-ALL with a myeloid phenotype when introduced into immature thymocytes. (A) A peripheral blood smear from leukemia induced by the Il7r 241-242TC mutant in a recipient mouse 64 d after transplant. Bar, 10 µm. (B) Co-expression of the vector (GFP) and the myeloid lineage marker Gr1 in leukemic cells from the spleen, BM, and peripheral blood as analyzed by flow cytometry. (C) Leukemic infiltrates were present in the kidney and other solid organs and tumor cells expressed myeloperoxidase (MPO) and GFP by immunohistochemistry. Bars, 75 µm. (D) PCR analysis for TCR rearrangements in ETP-ALL tumor cells. Two Il7r 241-242TC mutant ETP-ALL leukemic samples from the spleen (SP1, SP2) have monoclonal TCR rearrangements. Other controls show the unrearranged germline TCR in BM granulocytes and polyclonal TCR rearrangements (multiple bands) in normal DN thymocytes.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal