Chemokine expression and OPC population patterns throughout demyelination and remyelination. (A–E) Confocal IHC analysis of the CC of naive CXCR7^GFP/+ mice and after 3 or 6 wk of CPZ ingestion and after 10 d remyelination. Sections were stained for MBP (A), GFP (CXCR7; B), CXCR4 (C), CXCL12 (D), and NG2 (E). The mean positive area was analyzed for naive mice (N), mice fed CPZ for 3 or 6 wk, and mice fed CPZ for 6 wk and then a standard diet for a 10-d recovery period (R). (F and G) Confocal IHC analysis of the CC of CXCR7^GFP/+ mice after 3 wk (F) or 6 wk (G) of CPZ ingestion. Sections were stained for GFP (green) in combination with NG2, GFAP, Iba-1, CD31, and NFH (red). The area of co-localization was analyzed via Volocity image analysis software and normalized to the total GFP⁺ area. Representative images are shown and quantitative data were collected from 3 sections per mouse (n = 4–8 mice/group) across 2 independent experiments with results shown as fold change compared with naive ± SEM. Insets = isotype control (IC). Bars, 25 µm. *, P < 0.05; **, P < 0.01.