Figure 3.
Figure 3. CD40, BAFF-R, and BCR signaling of isolated splenic B cells. (A–C) CD43− B cells from the spleen of 9–10-wk-old control (Ptpn1f/f) and Ptpn1f/f-mb1cre mice were stimulated with 10 µg/ml anti-CD40 (A), 50 ng/ml recombinant mouse BAFF (B), or 10 µg/ml anti-IgM F(ab′)2 (C) for the given times. The activation of the indicated downstream signaling molecules was studied by Western blot. (Phosphorylated forms of the molecules are marked as “p-“.) The data shown are representative of three experiments with similar results. The molecular masses of the bands (in kilodaltons) are indicated in parentheses.

CD40, BAFF-R, and BCR signaling of isolated splenic B cells. (A–C) CD43− B cells from the spleen of 9–10-wk-old control (Ptpn1f/f) and Ptpn1f/f-mb1cre mice were stimulated with 10 µg/ml anti-CD40 (A), 50 ng/ml recombinant mouse BAFF (B), or 10 µg/ml anti-IgM F(ab′)2 (C) for the given times. The activation of the indicated downstream signaling molecules was studied by Western blot. (Phosphorylated forms of the molecules are marked as “p-“.) The data shown are representative of three experiments with similar results. The molecular masses of the bands (in kilodaltons) are indicated in parentheses.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal