Figure 3.
Figure 3. CARD9 mediates Curdlan-induced ERK activation but is not dispensable for NF-κB. (A and B) RAW264.7 cells stably expressing human Dectin-1, Dectin-2, or mock were stimulated with plate-coated curdlan (50 µg/ml) or α-mannans (40 µg/ml) for 1 h for preparing nuclear extract (A) or 30 min for preparing cell lysate (B), and then subjected to immunoblotting analysis using the indicated antibodies. (C–F) WT and CARD9-deficient BMDMs (C and E) or BMDCs (D and F) were stimulated with plate-coated curdlan (50 µg/ml) or α-mannans (40 µg/ml) for the indicated times for preparing cell lysate (C and D) and 1 h for preparing nuclear extract (E and F). The cell lysates or nuclear extracts were subjected to immunoblotting analysis using the indicated antibodies. Data shown are representative of three independent and reproducible experiments.

CARD9 mediates Curdlan-induced ERK activation but is not dispensable for NF-κB. (A and B) RAW264.7 cells stably expressing human Dectin-1, Dectin-2, or mock were stimulated with plate-coated curdlan (50 µg/ml) or α-mannans (40 µg/ml) for 1 h for preparing nuclear extract (A) or 30 min for preparing cell lysate (B), and then subjected to immunoblotting analysis using the indicated antibodies. (C–F) WT and CARD9-deficient BMDMs (C and E) or BMDCs (D and F) were stimulated with plate-coated curdlan (50 µg/ml) or α-mannans (40 µg/ml) for the indicated times for preparing cell lysate (C and D) and 1 h for preparing nuclear extract (E and F). The cell lysates or nuclear extracts were subjected to immunoblotting analysis using the indicated antibodies. Data shown are representative of three independent and reproducible experiments.

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