CARD9 engages the yeast-induced ERK, but not NF-κB activation. (A and B) WT and CARD9-deficient BMDMs (A) or BMDCs (B) were stimulated with UV-inactivated WT or mnn5 strains of C. albicans yeasts (MOI = 5) or hyphae (MOI = 1) for 1 h. Nuclear extracts were prepared and subjected to immunoblotting analysis using indicated antibodies. (C and D) WT and CARD9-deficient BMDMs (C) or BMDCs (D) were stimulated with UV-inactivated WT or mnn5 mutant strain of C. albicans yeast for the indicated times. Cell lysates were prepared and subjected to immunoblotting analysis using indicated antibodies. (E) WT and CARD9-deficient BMDMs were stimulated with UV-inactivated mnn5 yeast cells (MOI = 5) for the indicated times. Cell lysates were prepared and subjected to immunoblotting analysis using indicated antibodies. (F) WT and Bcl10-deficient BMDMs were stimulated with UV-inactivated WT or mnn5 yeast cells (MOI = 5) for the indicated times. Cell lysates were prepared and subjected to immunoblotting analysis using indicated antibodies. Data shown are representative of three independent and reproducible experiments.