Figure 3.
Figure 3. Decreased proliferation rate of embryo-derived cMΦ with age. (A) cMΦ subpopulations of adult CX3CR1GFP/+ mice were analyzed for BrdU incorporation and expression of Ki67 by flow cytometry four hours after BrdU injection (i.p.). Bars show median (n = 12–13). *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.005; Wilcoxon test. (B and C) Total (B) or CX3CR1+MHCII− (C) cMΦ of CX3CR1GFP/+ mice of indicated age were analyzed for BrdU incorporation and Ki67 expression by flow cytometry 4 h after BrdU injection (i.p.). Bars show median (n = 4–15). ***, P ≤ 0.005, Mann-Whitney test. Data in all panels were pooled from 2–3 independent experiments.

Decreased proliferation rate of embryo-derived cMΦ with age. (A) cMΦ subpopulations of adult CX3CR1GFP/+ mice were analyzed for BrdU incorporation and expression of Ki67 by flow cytometry four hours after BrdU injection (i.p.). Bars show median (n = 12–13). *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.005; Wilcoxon test. (B and C) Total (B) or CX3CR1+MHCII− (C) cMΦ of CX3CR1GFP/+ mice of indicated age were analyzed for BrdU incorporation and Ki67 expression by flow cytometry 4 h after BrdU injection (i.p.). Bars show median (n = 4–15). ***, P ≤ 0.005, Mann-Whitney test. Data in all panels were pooled from 2–3 independent experiments.

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