Silencing or Inhibition of ipak1 does not alter salp15, salp17, salp25D, β-tubulin, or gapdh gene expression. Q-RT-PCR showing levels of salp15 (A and B), salp17 (C and D), salp25D (E and F), β-tubulin (G and H), and gapdh (I and J) mRNA in mock and ipak1-dsRNA–injected ticks (A, C, E, G, and I) and in mock or PK-18– (PAK1 inhibitor) or Genistein (tyrosine kinase inhibitor)–injected ticks (B, D, F, H, and J). Mock controls were injected with buffer alone (A, C, E, G, and I) or DMSO (B, D, F, H, and J), respectively. The levels of transcripts were quantified against tick β-actin transcripts. Each circle represents an individual tick. Statistics were performed using the Student’s t test, and the p-value is shown. Horizontal bars in all panels represent mean values of the data points.