IL-22R expression in colonic epithelial cells in the Hh+AOM model. (A) Expression of IL-22R is restricted to colonic epithelial cells. Quantitative PCR analysis of mRNA levels of Il-22r and Il-10r2, the two subunits of IL-22 receptor, in colonic epithelial cells (EC), stromal cells (SC), and cLPs. Mean and SEM of one representative experiment (n = 3) of two is shown. (B) Expression of IL-22R in dysplastic epithelial cells of 5-mo Hh+AOM-treated mice as assessed by immunohistochemistry. *, P < 0.05; ***, P < 0.001; ****, P < 0.0001, one-way ANOVA with Bonferroni’s post test (A).