Figure 4.
Figure 4. Nkp46− CD4− lin− Thy1hi ILCs are the major source of IL-22 in Hh+AOM CRC. (A) Secretion of cytokines into the supernatant of overnight cultured cLP cells from uninfected or Hh+AOM-treated mice. (B) Intracellular cytokine stain gated on different ILC populations from uninfected or Hh+AOM-treated mice after 3 h culture of cLPs in the presence of monensin. (C–E) Characterization of IL-22 producers in cLPs of Hh+AOM-treated mice stimulated overnight in the presence of IL-23 and for 3 h with PMA, Ionomycin, and monensin (n = 5). (C) Intracellular stain with isotype (left) or anti–IL-22 (right). (D) Anti–IL-22 stains mostly Thy1hi (left) Nkp46− CD4− (right) cells. (E) IL-17 and IL-22 expression in Thy1hi cells. Data are shown as means and SEM. Results are representative of at least two independent experiments. *, P < 0.05, Mann-Whitney nonparametric test.

Nkp46− CD4− lin− Thy1hi ILCs are the major source of IL-22 in Hh+AOM CRC. (A) Secretion of cytokines into the supernatant of overnight cultured cLP cells from uninfected or Hh+AOM-treated mice. (B) Intracellular cytokine stain gated on different ILC populations from uninfected or Hh+AOM-treated mice after 3 h culture of cLPs in the presence of monensin. (C–E) Characterization of IL-22 producers in cLPs of Hh+AOM-treated mice stimulated overnight in the presence of IL-23 and for 3 h with PMA, Ionomycin, and monensin (n = 5). (C) Intracellular stain with isotype (left) or anti–IL-22 (right). (D) Anti–IL-22 stains mostly Thy1hi (left) Nkp46− CD4− (right) cells. (E) IL-17 and IL-22 expression in Thy1hi cells. Data are shown as means and SEM. Results are representative of at least two independent experiments. *, P < 0.05, Mann-Whitney nonparametric test.

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