Figure 3. NTHi can efficiently take up and incorporate Neu5Gc. (A) Dose-dependent uptake and expression of NTHi (2019) grown in a Sia-free defined media with 0.1 µM–1 mM Neu5Gc. Neu5Gc was detected by flow cytometry analysis using a chicken anti-Neu5Gc IgY antibody. (B) 2019 grown in 1 mM Neu5Gc were treated with sialidase (sialidase) or heat-inactivated sialidase (mock sialidase) and probed with a chicken anti-Neu5Gc IgY antibody in a whole-cell ELISA. Data are representative of greater than three independent experiments and show the mean of triplicate absorbance values at OD405. Error bars represent SD.
Figure 3.

NTHi can efficiently take up and incorporate Neu5Gc. (A) Dose-dependent uptake and expression of NTHi (2019) grown in a Sia-free defined media with 0.1 µM–1 mM Neu5Gc. Neu5Gc was detected by flow cytometry analysis using a chicken anti-Neu5Gc IgY antibody. (B) 2019 grown in 1 mM Neu5Gc were treated with sialidase (sialidase) or heat-inactivated sialidase (mock sialidase) and probed with a chicken anti-Neu5Gc IgY antibody in a whole-cell ELISA. Data are representative of greater than three independent experiments and show the mean of triplicate absorbance values at OD405. Error bars represent SD.

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