Ago2 regulates the expression of miRNAs in striatal neurons. (A) Ago2-dependent miRNAs. The bar graph displays miRNAs that are down-regulated in the striatum of Camk2a-Cre; Ago2^fl/fl mice as compared with Ago2^fl/fl control mice (n = 6/genotype). The miRNAs are ordered according to the degree of their down-regulation. (B) Ago2-dependent 3p miRNAs are enriched for A/U at their 5′ position. Logo diagrams display all 3p Ago2-dependent and 3p Ago2-independent miRNAs in the striatum of Camk2a-Cre; Ago2^fl/fl mice as compared with Ago2^fl/fl control mice (n = 6/genotype). The height of each row corresponds to the sequence conservation at specific positions, and the height of individual letters within a row corresponds to the prevalence of that specific nucleotide (nt). Sequences ranging from 30 nt upstream to 48 nt after the first nucleotide of the mature miRNA sequence are depicted. The 5′-nt of the mature miRNA sequence is located at position 0 (indicated by the beginning of the red bar). 3p miRNAs down-regulated in the striatum of Camk2a-Cre; Ago2^fl/fl (top) show higher occurrence of A or U at the 5′-nt of the mature miRNA than 3p miRNAs that are unaffected by the loss of Ago2 (bottom). The table displays the percentage of Ago2-dependent (left) and Ago2–independent (right) 3p miRNAs that have an A/U versus a C/G at their 5′-position.