![Figure 1. T cell hybridomas recognize native LDL and its ApoB100 protein. (A) 105 hybridoma cells from each of 23 different clones were incubated with 4 × 105 irradiated APCs with 20 µg /ml of LDL, oxLDL, or ApoB100. Each column represents one clone. Media without LDL were used as negative controls. Results are from one experiment. (B–D) 105 hybridoma cells of three different clones (B, hybridoma 15–2; C, 48–5; D, 45–1) were incubated with 4 × 105 irradiated APCs and different concentrations of purified human ApoB100 from plasma LDL, or transgenic human ApoB100 from huB100tg x Ldlr−/− mice. Hybridomas 15–2, 48–5, and 45–1 (B–D) recognize purified and transgenic ApoB100 in a dose-dependent manner. In all experiments, IL-2 secretion was used as a measure of activation. Data show means ± SEM. Results are representative of three independent experiments. (E–G) 105 hybridoma cells were incubated with 4 × 105 irradiated APCs with 20 µg/ml of LDL or LDL oxidized to different extents (copper oxidation [20 µM CuSO4] for varying lengths of time). After 24 h of incubation with the different preparations, IL-2 secretion was measured in the supernatant. X axis shows the mean of TBARS values and y axis shows the mean of IL-2 levels. All T cell hybridomas showed an inverse correlation between IL-2 levels and the degree of LDL oxidation. Results are representative of three independent experiments.](https://cdn.rupress.org/rup/content_public/journal/jem/207/5/10.1084_jem.20092243/4/jem_20092243_lw_fig1.jpeg?Expires=1768786264&Signature=3tyl23puzJyev~Kkk~wXd3tzbjBs1cmj8cMpDSHl0fbNdByG7KdKCmwRraK-8lxijRJp9M9zq6o3vEWG6eMPFLrPSQgbvJn9~t8UzfwalJm9gv2dtD7UCNWEdtxOBeR7UtdJ14x5AwLnQLL7NuX8K92IMIJqLC~Bk0mVdf9PbeyS5hwauhtsuUstWn922psybIK~zzP-lV5IikAlcau5pblAMa9mwWWT33WXcxDYg0~96ll8LS2HBa0NME~Eax21snOG4mWoA5Sp3dR7QaySI6bhNDgwcWhU4ik7jifgTqwI5dKUjqY7bUBYUWfrdjEp6FUUo~lV0hx3SGspu~Pv7g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
T cell hybridomas recognize native LDL and its ApoB100 protein. (A) 105 hybridoma cells from each of 23 different clones were incubated with 4 × 105 irradiated APCs with 20 µg /ml of LDL, oxLDL, or ApoB100. Each column represents one clone. Media without LDL were used as negative controls. Results are from one experiment. (B–D) 105 hybridoma cells of three different clones (B, hybridoma 15–2; C, 48–5; D, 45–1) were incubated with 4 × 105 irradiated APCs and different concentrations of purified human ApoB100 from plasma LDL, or transgenic human ApoB100 from huB100tg x Ldlr−/− mice. Hybridomas 15–2, 48–5, and 45–1 (B–D) recognize purified and transgenic ApoB100 in a dose-dependent manner. In all experiments, IL-2 secretion was used as a measure of activation. Data show means ± SEM. Results are representative of three independent experiments. (E–G) 105 hybridoma cells were incubated with 4 × 105 irradiated APCs with 20 µg/ml of LDL or LDL oxidized to different extents (copper oxidation [20 µM CuSO4] for varying lengths of time). After 24 h of incubation with the different preparations, IL-2 secretion was measured in the supernatant. X axis shows the mean of TBARS values and y axis shows the mean of IL-2 levels. All T cell hybridomas showed an inverse correlation between IL-2 levels and the degree of LDL oxidation. Results are representative of three independent experiments.
