Fusion and infectivity. (A) (left) Histogram plots summarize fusion between BOSC.23 cells expressing GFP alone (w/o) or BaL gp160Δc or BaL gp160ΔcD474A/M475A/R476A with mCherry expressing TZM.bl cells. (right) Fusion events (%) with mean and standard deviation for at least two independent experiments. (B) Image-based analysis of fusion. (left) Single staining compensation controls for BOSC.23 cells expressing BaL gp160Δc and GFP, mCherry expressing TZM.bl cells and CD4-APC stained TZM.bl cells. (middle) An intermediate fusion event. (right) The colocalization of the GFP-mCherry-APC signal after fusion. Experiment has been performed twice. (C) Graphs show the titration curves for TZM.bl cell infection by YU2 and BaL pseudoviruses, which has been performed twice. RLU relative light unit.
