NLR inflammasome components attenuate recurring ulcerative colitis. (A) Schematic of the recurring model of DSS-induced ulcerative colitis. (B–D) Weight loss of Pycard^−/−, Nlrp3^−/−, and Casp1^−/− mice after DSS challenge. (E) Clinical parameters (weight loss, stool consistency, and bleeding) of indicated mice. These data are the averages of each clinical score collected throughout the recurring colitis model. (F) Colon length of indicated mice. (G) Colon histopathology scores reflecting the severity of inflammation, epithelial defects, crypt atrophy, and area affected in hematoxylin and eosin–stained sections through the mid and distal colon. The individual scores are shown in Fig. S2 and summed to generate a HAI. Colon length and histopathology were assessed in the Casp1^−/− animals on day 40, whereas these two parameters were assessed on day 57 in the other genotypes tested. Data shown are representative of three independent experiments and depict the mean ± SEM. Error bars have been omitted from the weight loss data for clarity of presentation. The symbols # and § indicate P < 0.05 and P < 0.01, respectively, between the mock- and DSS-treated WT; * indicates P < 0.05 between the DSS-treated gene-deficient strains and WT. WT mock, n = 5; WT, n = 5; Pycard^−/−, n = 3; Casp1^−/−, n = 4; Nlrp3^−/−, n = 3.