Modulations of miR-612 on EMT through AKT2 pathway. (A) Image showing the morphological changes of HCCLM3 after indicated treatment at hour 72. (B) Real-time PCR and Western blot analysis to quantify the mRNA and protein levels of E-cadherin (E-cad) and Vimentin (VIM) in HCCLM3 cells. β-Actin was a loading control, and the amount was normalized by WT cells in PCR experiments (WT). (C) Image showing the morphological changes of HepG2 after treatment with indicated reagent at hour 72. (D) Real-time PCR and Western blot analysis to quantify the mRNA and protein levels of E-cadherin (E-cad) and Vimentin (VIM) in HepG2 cells. β-Actin was used as a loading control, and the amount was normalized by WT cells in PCR experiments (WT). WT, mock, miR-612-o, miR-612-i, AKT2-o, or AKT2-i were defined as nontransfected, negative control oligonucleotide transfected, miR-612 mimic transfected, miR-612 inhibitor transfected, AKT2 overexpressed, or AKT2 knocked-down, respectively. Bars: (A) 100 µm; (C) 200 µm. Data are mean ± SEM (n = 3) and are representative of three independent experiments.