Figure 1.
Figure 1. G9a is a critical component of Th cell differentiation. CD4+ Th cells isolated from G9afl/fl and G9a−/− mice were stimulated for 6 d under neutral, Th1, and Th2 conditions and analyzed by immunoblotting for G9a, GLP, H3K9me2, and β-actin (A), qPCR for mRNA levels of G9a (B), ChIP for H3K9me2 (C), intracellular cytokine staining for IL-13 and IFN-γ production by CD4+ T cells by flow cytometry (D), ELISA for IL-13 and IFN-γ (E), qPCR for mRNA levels of Il13 and Ifng (F), and qPCR for expression of Stat4, Tbx21, Stat6, and Gata3 (G). Results are representative of six independent experiments. Rel. units, relative units. ND, not detected. Asterisks indicate P < 0.05. Error bars indicate SEM.

G9a is a critical component of Th cell differentiation. CD4+ Th cells isolated from G9afl/fl and G9a−/− mice were stimulated for 6 d under neutral, Th1, and Th2 conditions and analyzed by immunoblotting for G9a, GLP, H3K9me2, and β-actin (A), qPCR for mRNA levels of G9a (B), ChIP for H3K9me2 (C), intracellular cytokine staining for IL-13 and IFN-γ production by CD4+ T cells by flow cytometry (D), ELISA for IL-13 and IFN-γ (E), qPCR for mRNA levels of Il13 and Ifng (F), and qPCR for expression of Stat4, Tbx21, Stat6, and Gata3 (G). Results are representative of six independent experiments. Rel. units, relative units. ND, not detected. Asterisks indicate P < 0.05. Error bars indicate SEM.

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