Transgenic expression of PLUNC in lungs of Nogo-A/B KO mice reduces allergic Th2 inflammation. (A) Lung sections from WT, KO, PLUNC transgenic (PTG)–WT, and PTG-KO were stained for PLUNC. Endogenous PLUNC (only in the trachea and upper airways) was observed in tracheal epithelium of WT and, to a lesser extent, in KO (top). Transgenic PLUNC was observed in epithelium of upper airways (bottom) as well as further down the smaller airways (insets) in both WT and KO backgrounds. (B) BAL from WT, PTG-WT, KO, and PTG-KO mice with and without OVA challenge were immunoblotted for levels of endogenous PLUNC (mPLUNC at 25 kD) and transgenic PLUNC (hPLUNC at 20 kD) protein expression. Each lane of the Western blot represents lung lysate from typical individual mouse and was observed in n = 4–8 mice for each group. (C and D) Lung H&E staining (C) and total and differential BAL cell counts (D) of KO and PTG-KO mice challenged with OVA (*, P < 0.05; **, P < 0.01). Bars, 200 µm. Data are expressed as the mean ± SEM. n = 6 mice for both groups representative of two experiments.