Deficiency in IκBβ reduces LPS-triggered production of sepsis inducing cytokines. (A) Serum levels of IL-1β, TNF, and IL-6 in IκBβ^−/− and control mice after LPS injection (30 mg/kg). TNF, IL-1β, and IL-6 were measured in serum collected from tail vain 0, 1, 2, and 6 h after injection of LPS (Student’s t test; *, P < 0.001 versus controls). (B) Liver IL-1β, TNF, and IL-6 mRNA expression levels after LPS challenge (30 mg/kg). At the indicated time points after LPS injection (30 mg/kg), total RNA from whole livers were prepared and mRNA levels were quantified using real-time PCR analysis (Student’s t test; *, P < 0.05 versus controls). For each time point, four animals per strain were examined in two independent experiments.