Ldb1 complexes regulate expression of erythroid, megakaryocytic, and prosurvival genes. Stable clones of MEL cells expressing ldb1 shRNA or control shRNA were treated with 1.5% DMSO to induce erythroid differentiation. Total RNA was isolated at the indicated times and gene expression was quantified by real-time RT-PCR with β-actin as control. (A) Expression of genes encoding Ldb1 complex subunits and erythroid/megakaryocytic lineage proteins are shown. (B) Expression of prosurvival genes (bcl-xl, eopr, and sox6) in MEL cells expressing ldb1 shRNA or control shRNA after induction with DMSO. Results shown are representative of two independent induction experiments. Values are expressed as means ± SD.