![Figure 2. Absence of long-term repopulating HSC activity and HSC generation in +9.5−/− AGM. (A) Contribution from +9.5+/− and +9.5−/− versus +9.5+/+ AGM explants (in a competitive transplantation assay +9.5+/+ [n = 11]; +9.5+/− [n = 11]; +9.5−/− [n = 6]). (B) Secondary transplant with bone marrow cells from primary recipient mice (+9.5+/+ [n = 8]; +9.5+/− [n = 8]). (C) The contribution of +9.5+/+ and +9.5+/− donor-derived CD45.2 cells to peripheral myeloid cells (Mac1+), B cells (CD19+) and T cells (Thy1.2+). (D) Whole-mount immunostaining of E10.5 +9.5+/+ (left) and +9.5−/− (right) embryos showing CD31+ cells (magenta) and c-Kit+ cells (green) within the aorta region. Bar, 100 µm. (E) Higher magnification of boxed region in D showing the representative aortic hematopoietic clusters in +9.5+/+ embryos. Bar, 20 µm. (F) Quantitation of c-Kit+ cells within the whole dorsal aorta (2 litters: +9.5+/+ [n = 3]; +9.5−/− [n = 3]). Error bars represent SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (two-tailed unpaired Student’s t test). Related to Videos 1 and 2.](https://cdn.rupress.org/rup/content_public/journal/jem/210/13/10.1084_jem.20130733/4/jem_20130733r_fig2.jpeg?Expires=1767769271&Signature=algoIz8~hVnovOcCl5khKAB9TxWUNgY4Mquv2oF1MiwGOZ8WoK~yWAcZznsLetVm6PIjmAbBaQ2XxcVFLhh7uMgZenusQRL2e9QvuJ2jSk4de6IKQwqonYYTrPUqhk8D2QhDgroFyVcsRe4HT4UVj8dNwqfWffVYDv74RXa3WvjY7Gk6nhsogkPrAuE4HywGzS8o40OvLPC9HTk6KAGn6mSJwKwQL6dUkHJqpbByOV-NZgS6LOXD9biiL5FRRUJJoCxu-Tpm4IRH~5Z5f8JCmgy63BGFB0DaCkxtFsbf0fl2X8SVMNLL370BYYfhkFn5HkQZC6pLr63Ifvl5HjokAg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Absence of long-term repopulating HSC activity and HSC generation in +9.5−/− AGM. (A) Contribution from +9.5+/− and +9.5−/− versus +9.5+/+ AGM explants (in a competitive transplantation assay +9.5+/+ [n = 11]; +9.5+/− [n = 11]; +9.5−/− [n = 6]). (B) Secondary transplant with bone marrow cells from primary recipient mice (+9.5+/+ [n = 8]; +9.5+/− [n = 8]). (C) The contribution of +9.5+/+ and +9.5+/− donor-derived CD45.2 cells to peripheral myeloid cells (Mac1+), B cells (CD19+) and T cells (Thy1.2+). (D) Whole-mount immunostaining of E10.5 +9.5+/+ (left) and +9.5−/− (right) embryos showing CD31+ cells (magenta) and c-Kit+ cells (green) within the aorta region. Bar, 100 µm. (E) Higher magnification of boxed region in D showing the representative aortic hematopoietic clusters in +9.5+/+ embryos. Bar, 20 µm. (F) Quantitation of c-Kit+ cells within the whole dorsal aorta (2 litters: +9.5+/+ [n = 3]; +9.5−/− [n = 3]). Error bars represent SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (two-tailed unpaired Student’s t test). Related to Videos 1 and 2.
