Inflammatory LCs express PU.1, ID2, and Langerin. (A and B) Donor cells (Ly5.2⁺) from either PU.1^GFP and ID2^GFP (A) or wild-type (wt) and ID2cKO mice (B) were subjected to the same experimental regimen outlined in Fig. 5 A. (A) Expression of GFP within the CD11c⁻MHCII⁻ (black line), CD11c⁺MHCII⁻ (filled blue), and CD11c⁺MHCII⁺ inflammatory LC (filled red) fractions was determined by flow cytometry. (B) BM-derived LCs from wild-type or ID2cKO donors (Ly5.2⁺) were analyzed for expression of CD11c, Langerin, and MHCII by flow cytometry. Expression of MHCII within the CD11c⁻Langerin⁻ (black line), CD11c⁺Langerin⁻ (filled blue), and CD11c⁺Langerin⁺ (filled red) fractions was determined by flow cytometry. (C) Leukocytes from ID2cKO donors (Ly5.2⁺) were analyzed for expression of CD11c and MHCII by flow cytometry. Gated Ly5.2⁺CD11c⁻MHCII⁻ (gate I) and Ly5.2⁺CD11c⁺MHCII⁻ (gate II) were examined for TCR-β and TCR-γδ by flow cytometry. Data in this figure are representative of two independent experiments.