Characterization of the expression of PU.1 and ID2 in circulating monocytes. (A) Circulating mononuclear cells were isolated from blood and analyzed by flow cytometry. CD11b⁺MCSFR⁺ monocytes (left plot) were gated and the expression of Ly6C and CD11c was analyzed (right plot). Histograms represent the expression of CX3CR1 using CX3CR1^GFP reporter mouse on inflammatory CD11c⁻ly6C^high monocytes (dashed green histogram) and resident CD11c⁺ly6C^-/low monocytes (green filled histogram). (B and C) The mean of frequency of total CD11b⁺MCSFR⁺ circulating monocytes (B) and CD11b⁺MCSFR⁺ CD11c⁻Ly6C^high inflammatory monocytes (C) are shown. The data are the mean ± SD from five mice. (D) Reporter mice of the indicated genotypes were assessed for the expression of GFP in in the CD11b⁺MCSFR⁺CD11c⁻Ly6C^high (dashed green line) and CD11b⁺MCSFR⁺ CD11c⁺Ly6C^−/low (filled green line) compartments. Gray histograms show autofluorescence in identical populations isolated from wild type blood. Numbers represents the MFI of GFP for the indicated population ± SD. Data are representative of two independent experiments.