Figure 6.
Figure 6. Nle is required for the large preribosomal subunit biogenesis in mouse ES cells. (A) Western blot analysis on NlecKO cells untreated or treated with 4-OHT. This experiment was performed three times independently. (B) Immunostaining of NLE and B23 in Rosa26CreERT2/+; NleFlox/null ES cells treated or not with 4-OHT. The pictures are representative of three independent experiments. Bars, 10 µm. (C) RNA profiles measured by absorbance at 260 nm in a sucrose gradient loaded with extracts from untreated cells. In each fraction, NLE and RPL24 protein levels, 28S and 18S rRNA, were assessed by immunoblotting and ethidium bromide staining, respectively. The arrow points to the NLE-specific band. (D) RNA profiles measured by absorbance at 260 nm loaded with extracts from untreated and treated cells at day 3. Similar results were obtained for two independent sedimentation profile experiments.

Nle is required for the large preribosomal subunit biogenesis in mouse ES cells. (A) Western blot analysis on NlecKO cells untreated or treated with 4-OHT. This experiment was performed three times independently. (B) Immunostaining of NLE and B23 in Rosa26CreERT2/+; NleFlox/null ES cells treated or not with 4-OHT. The pictures are representative of three independent experiments. Bars, 10 µm. (C) RNA profiles measured by absorbance at 260 nm in a sucrose gradient loaded with extracts from untreated cells. In each fraction, NLE and RPL24 protein levels, 28S and 18S rRNA, were assessed by immunoblotting and ethidium bromide staining, respectively. The arrow points to the NLE-specific band. (D) RNA profiles measured by absorbance at 260 nm loaded with extracts from untreated and treated cells at day 3. Similar results were obtained for two independent sedimentation profile experiments.

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