Figure 5. Chemotherapy stimulates CAF cytokine secretion. Cytokine secretion of CAFs treated with vehicle (DMSO) or with chemotherapy for 3 d was assessed using a 120 human cytokine antibody array from RayBio. (A–C) CAFs derived from three human surgical specimens and one commercial normal intestinal fibroblast line were interrogated for their cytokine and chemokine profile after chemotherapy treatment. (B) HGF was not significantly up-regulated in our cytotoxin conditions compared with vehicle. (C) The mean concentrations of DTK, IL-17A, and TGFβ were statistically significant in the three CAFs treated with chemotherapy compared with vehicle. (n = 1); P < 0.05. Student’s t test was used to assess the significance. The experiment was performed once. (D) Quantitative RT-PCR analysis of DTKs, IL-17A, and TGFβ was performed to validate the cytokine array results. Data are presented as mean ± SD (n = 3); *, P < 0.05. Student’s t test was used to assess the significance. The experiment was performed three times and representative data are shown. (E) Frozen sections of human colorectal tumors matched before and after cytotoxic treatment from two patients were co-stained for IL-17A and αSMA. Bars, 50 µm.
Figure 5.

Chemotherapy stimulates CAF cytokine secretion. Cytokine secretion of CAFs treated with vehicle (DMSO) or with chemotherapy for 3 d was assessed using a 120 human cytokine antibody array from RayBio. (A–C) CAFs derived from three human surgical specimens and one commercial normal intestinal fibroblast line were interrogated for their cytokine and chemokine profile after chemotherapy treatment. (B) HGF was not significantly up-regulated in our cytotoxin conditions compared with vehicle. (C) The mean concentrations of DTK, IL-17A, and TGFβ were statistically significant in the three CAFs treated with chemotherapy compared with vehicle. (n = 1); P < 0.05. Student’s t test was used to assess the significance. The experiment was performed once. (D) Quantitative RT-PCR analysis of DTKs, IL-17A, and TGFβ was performed to validate the cytokine array results. Data are presented as mean ± SD (n = 3); *, P < 0.05. Student’s t test was used to assess the significance. The experiment was performed three times and representative data are shown. (E) Frozen sections of human colorectal tumors matched before and after cytotoxic treatment from two patients were co-stained for IL-17A and αSMA. Bars, 50 µm.

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