Deficit of naive T cells in the peripheral lymphoid organs of Aif-hypomorphic Hq mice. (A) Representative two-color analyses of splenic and lymph node cells from 8–12-wk-old WT and Hq mice stained for CD4 and CD8, showing frequencies of cells in quadrants. (B–D) Frequencies (B) and absolute numbers of CD4 and CD8 cells in the spleen (C) and inguinal lymph node (D) of WT and Hq mice. *, P < 0.01. (E) Absolute numbers of B220⁺ B cells (B) and CD11b⁺ macrophages (M) in the spleen (S) and inguinal lymph node (LN) of WT and Hq mice. (F) Representative analysis of CD44 levels on gated CD4 or CD8 cells from spleen or lymph nodes of WT (thin lines) or Hq (thick lines) mice. Filled histograms represent isotype controls. (G) Absolute numbers of CD44^lo (naive) and CD44^hi (memory) CD4 and CD8 cells per organ in the spleen (S) and inguinal lymph nodes (LN) of WT and Hq mice. *, P < 0.01; **, P < 0.05. All data are shown as mean ± SE (n = 3–5) and are representative of at least three independent experiments.