Flt3L treatment exacerbates pancreatitis. (A) The time course of DC expansion in the pancreas of Flt3L-treated WT mice is shown. Both the total number of pancreatic DCs and their fraction among pancreatic leukocytes are indicated. Data are based on a mean of four mice per time point. (B) The fraction of inflammatory monocytes in the pancreata of Flt3L-treated mice and controls was calculated. Day 15 data are shown. (C and D) Mice treated with Flt3L alone, caerulein alone, or caerulein + Flt3L were stained with H&E, Gomori’s Trichrome, and anti-CD45. (D) Fibroinflammatory area was quantified by examining 10 HPFs per mouse (five mice per group; ***, P < 0.001). Error bars indicate standard error of the mean.