Figure 4. Exogenously added antibodies specific for CD36 are transported into the VCC. (A) Confocal micrographs of HIV-1 Gag-iGFP–infected primary macrophages are presented. Cells were exposed for 2 h at 37 or 4°C with the indicated mAb, then fixed and stained for CD9, whereas isotype control mAbs as well as CD36-specific mAbs were revealed with appropriate secondary antibodies (Bars, 10 µM). (B) Confocal micrographs of HIV-1 Gag-iGFP–infected macrophages simultaneously exposed to antibodies specific for SR-BI (rabbit antibodies) and CD36 (mouse mAb) for 2 h. Both types of antibodies are internalized at 37°C (not at 4°C), but only the CD36-specific mAb colocalizes with Gag+ compartments. Merge images include DAPI staining in blue. Data shown are representative of two independent experiments. Bars, 10 µM.
Figure 4.

Exogenously added antibodies specific for CD36 are transported into the VCC. (A) Confocal micrographs of HIV-1 Gag-iGFP–infected primary macrophages are presented. Cells were exposed for 2 h at 37 or 4°C with the indicated mAb, then fixed and stained for CD9, whereas isotype control mAbs as well as CD36-specific mAbs were revealed with appropriate secondary antibodies (Bars, 10 µM). (B) Confocal micrographs of HIV-1 Gag-iGFP–infected macrophages simultaneously exposed to antibodies specific for SR-BI (rabbit antibodies) and CD36 (mouse mAb) for 2 h. Both types of antibodies are internalized at 37°C (not at 4°C), but only the CD36-specific mAb colocalizes with Gag+ compartments. Merge images include DAPI staining in blue. Data shown are representative of two independent experiments. Bars, 10 µM.

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