Figure 6. CDK inhibition decreases... | Rockefeller University Press

Figure 6.

$Figure 6. CDK inhibition decreases colony size in triple-negative cancer cells in 3D Matrigel matrices. (A) Low- and high-magnification images of 3D Matrigel cultures of T47D, HCC1428, BT549, and SUM149 cell lines at day 0 of treatment and day 6 of DMSO-treated, purvalanol A–treated, and dinaciclib-treated cultures. Nonrefractile dark particles indicated the presence of cell death. Bars, 10 µm. (B) Quantification of colony size of 3D Matrigel cultures. Low-magnification images of Matrigel cultures were obtained, and the mean size of the colonies in each culture was determined both at the initial day of treatment (day 0) and at the final day of treatment (day 6). The mean colony size at day 0 was set to 100% for each cell line and the colony size at day 6 was compared with the day 0 size. The experiment was independently repeated three times. The error bars represent means ± SEM. P-values were calculated by two-tailed Student’s t test.$

CDK inhibition decreases colony size in triple-negative cancer cells in 3D Matrigel matrices. (A) Low- and high-magnification images of 3D Matrigel cultures of T47D, HCC1428, BT549, and SUM149 cell lines at day 0 of treatment and day 6 of DMSO-treated, purvalanol A–treated, and dinaciclib-treated cultures. Nonrefractile dark particles indicated the presence of cell death. Bars, 10 µm. (B) Quantification of colony size of 3D Matrigel cultures. Low-magnification images of Matrigel cultures were obtained, and the mean size of the colonies in each culture was determined both at the initial day of treatment (day 0) and at the final day of treatment (day 6). The mean colony size at day 0 was set to 100% for each cell line and the colony size at day 6 was compared with the day 0 size. The experiment was independently repeated three times. The error bars represent means ± SEM. P-values were calculated by two-tailed Student’s t test.

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