Figure 7. The G159A allele exerts a dominant-negative effect, whereas the D50A allele does not. (a) IFN-β and IFN-λ production, as assessed by ELISA, in response to 50 µg/ml poly(I:C) stimulation or 25 µg/ml poly(I:C) transfection mediated by Lipofectamine (Lipo) in TLR3-deficient fibroblasts and in control fibroblasts (C+) transduced with lentivirus pseudoparticles encoding luciferase, WT TBK1, or one of the TBK1 mutants (G159A or D50A) or left untransduced (NT). Cells were transduced 6 d before stimulation. This experiment is representative of three independent experiments. NS, nonstimulated. (b) Western blot of transduced control fibroblasts (C+) with anti-TBK1 and antitubulin antibodies.
Figure 7.

The G159A allele exerts a dominant-negative effect, whereas the D50A allele does not. (a) IFN-β and IFN-λ production, as assessed by ELISA, in response to 50 µg/ml poly(I:C) stimulation or 25 µg/ml poly(I:C) transfection mediated by Lipofectamine (Lipo) in TLR3-deficient fibroblasts and in control fibroblasts (C+) transduced with lentivirus pseudoparticles encoding luciferase, WT TBK1, or one of the TBK1 mutants (G159A or D50A) or left untransduced (NT). Cells were transduced 6 d before stimulation. This experiment is representative of three independent experiments. NS, nonstimulated. (b) Western blot of transduced control fibroblasts (C+) with anti-TBK1 and antitubulin antibodies.

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