Impaired IFN-dependent control of HSV-1 and VSV infection in the patients’ fibroblasts. (a and b) IFN-β and IFN-λ production, as measured by ELISA, in the patients’ fibroblasts, control cells (C+; averaged from two distinct control cell lines), and UNC-93B^−/− and NEMO^−/− fibroblasts after 24 h of stimulation with HSV-1 (a) and 30 min or 24 h of stimulation with VSV (b). The graphs display means ± SD determined from three independent experiments. (c and d) Replication of the HSV-1–GFP virus at an MOI of 1 (c) and of VSV at an MOI of 10 (d) in the patients’ fibroblasts (P1 and P2) and in control cells (C+; averaged from two distinct control cell lines), and UNC-93B^−/− and STAT-1^−/− fibroblasts, as determined at the indicated hours after infection, with or without 18-h IFN-α2b pretreatment. One experiment representative of two independent experiments performed is shown. (e) Viability of control cells (C+; averaged from two different control cell lines) and in fibroblasts from P1, an UNC-93B^−/− patient, and a STAT-1^−/− patient after infection with HSV-1–GFP at MOIs of 0.1 and 0.5, with or without IFN-α2b treatment 18 h before infection. (f) Cell mortality after 24 h of VSV infection in control cells (C+; averaged from two distinct control cell lines) and in fibroblasts from P1, P2, a UNC-93B^−/− patient, and a STAT-1^−/− patient, with or without 18-h IFN-α2b pretreatment. NS, nonstimulated.