Figure 5. Genome-wide transcriptional evaluation of the TLR3 pathway in primary fibroblasts. (a) Cumulative fold change (FC) score (top) and heat maps (bottom) of the transcripts regulated by 8 h of stimulation with poly(I:C) (left) or IFN-α2b (right) in primary fibroblasts from three healthy controls (C+), both TBK1 patients (P1 and P2), a patient with TLR3 AR deficiency (TLR3−/−), a patient with AD TLR3 deficiency (AD TLR3), and a patient with STAT-1 complete deficiency (STAT1−/−). The cumulative score is the sum of all the fold change values of >1.5 (up- or down-regulation). Heat maps show a hierarchical clustering of transcripts differentially expressed upon stimulation (based on 100 differences in intensity and 1.5-fold changes compared with nonstimulated condition in healthy controls). Changes with respect to the unstimulated condition are shown by a color scale: red, up-regulated; blue, down-regulated; yellow, no change. The probes displaying differences of >100 in intensity were used to calculate the cumulative score. (b) Ranking of the 112 transcripts up-regulated after 8 h of poly(I:C) stimulation, with a fold change of at least 1.5 in all three controls tested, in primary fibroblasts from three healthy controls averaged together (Average C+), both TBK1 patients (P1 and P2), a patient with TLR3 AR deficiency (TLR3−/−), a patient with AD TLR3 deficiency (AD TLR3), and a patient with STAT-1 complete deficiency (STAT1−/−).
Figure 5.

Genome-wide transcriptional evaluation of the TLR3 pathway in primary fibroblasts. (a) Cumulative fold change (FC) score (top) and heat maps (bottom) of the transcripts regulated by 8 h of stimulation with poly(I:C) (left) or IFN-α2b (right) in primary fibroblasts from three healthy controls (C+), both TBK1 patients (P1 and P2), a patient with TLR3 AR deficiency (TLR3−/−), a patient with AD TLR3 deficiency (AD TLR3), and a patient with STAT-1 complete deficiency (STAT1−/−). The cumulative score is the sum of all the fold change values of >1.5 (up- or down-regulation). Heat maps show a hierarchical clustering of transcripts differentially expressed upon stimulation (based on 100 differences in intensity and 1.5-fold changes compared with nonstimulated condition in healthy controls). Changes with respect to the unstimulated condition are shown by a color scale: red, up-regulated; blue, down-regulated; yellow, no change. The probes displaying differences of >100 in intensity were used to calculate the cumulative score. (b) Ranking of the 112 transcripts up-regulated after 8 h of poly(I:C) stimulation, with a fold change of at least 1.5 in all three controls tested, in primary fibroblasts from three healthy controls averaged together (Average C+), both TBK1 patients (P1 and P2), a patient with TLR3 AR deficiency (TLR3−/−), a patient with AD TLR3 deficiency (AD TLR3), and a patient with STAT-1 complete deficiency (STAT1−/−).

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