Identification of DC subsets in human axillary LNs. (A) Axillary LN cells were enriched for DCs and stained for HLA-DR, CD11c, CD1a, CD14, EpCAM, BDCA4, BDCA1, Clec9A, and CD206. pDC, CD14⁺, CD1a⁺, Clec9A⁺, CD206⁺, BDCA1⁺ cell subsets and LCs were gated as depicted. Representative results of 12 independent experiments are shown. (B) DCs were allowed to migrate out of skin explants for 48 h and stained for HLA-DR, CD11c, CD1a, CD14, EpCAM, BDCA1, Clec9A, and CD206. Representative results of five independent experiments are shown. The percentage of each population among HLA-DR⁺ cells and mean ± SD in five donors are shown. (C and D) Purified LN cell populations were submitted to cytospin and Giemsa/May-Grünwald staining. Representative images are shown of three to four independent experiments. (D) CD14⁺ LN cells were stained for CD163. Gray histograms represent control isotype staining. Representative results of five independent experiments are shown. Bars, 10 µm. (E and F) LN cells were incubated with fluorescently labeled apoptotic cells at 4°C or 37°C. (E) Representative results of four independent experiments are shown. (F) Quantification of capture was performed by subtracting the MFI value at 4°C from the MFI value at 37°C. (G) Percentage of each population among lineage (Lin)⁻ HLA-DR⁺ cells and among Lin⁻ HLA-DR⁺CD11c⁺CD14⁻ cells. Mean ± SD in 18 donors is shown.