PLCγ2 deficiency leads to increased PMN- and MO-MDSCs in tumor-bearing mice. (A and B) Bone marrow and spleens from tumor-free (A, No Tumor) or LLC tumor–bearing (B, Tumor) WT and PLCγ2^−/− mice were harvested, and single-cell suspensions were stained for Ly6G and Ly6C markers. Percentages of WT and PLCγ2^−/− PMN-MDSCs (CD11b⁺Ly6G⁺Ly6C^low) and MO-MDSCs (CD11b⁺Ly6G^-Ly6C^high) are shown. Data are expressed as mean ± SD (n = 7). Cumulative results from two experiments are shown (A and B). *, P < 0.05; **, P < 0.01.