![Figure 5. Induction of the PC transcriptional program is intact in IL-21–stimulated STAT3MUT memory B cells. (A and B) Naive (CD20+CD10−CD27−IgG−; A) and memory (CD20+CD10−CD27+; B) B cells were sort-purified from normal donor controls (Ctl; n = 10 [or 7 for AICDA]), STAT3MUT patients (n = 5), or STAT1MUT patients (n = 4) and then cultured with CD40L alone or together with IL-21 (+IL-21) for 5 d. Expression of PAX5, PRDM1, XBP1, IRF4, and AICDA was determined by qPCR. The columns represent the mean ± SEM of experiments performed using naive B cells from 7–10 normal donors, 5 STAT3MUT patients, or 4 STAT1MUT patients. Levels of expression are relative to the amount of GAPDH. ns, not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001.](https://cdn.rupress.org/rup/content_public/journal/jem/210/12/10.1084_jem.20130323/5/jem_20130323_fig5.jpeg?Expires=1767686773&Signature=Nd59NMDSzbNsDAqRLmuJufrVcmq4rSF5WUvc90-kAAn~UeCAtZzUNWTm1ljrzSzYY9Xl9nhUFzqU7HyEbR0nTRLYn21X44lYW-tbF-hW8De3M6XHb5bW-yi0W05NzLql~72LkaXnte6Vv9qvXh0A07FaL1CBIqzUIMTMYPODzrzneJ37crh5YcNhoC2rR5xBvCMKh7qTuVcov33bsRYmpbxqI0W0uXFgfx68OnoU9NWoft4iIoxl4Pzj1AfZ6NvrysLh5lRX4HqfIaTNMDi1Je2QjI74JWNhqDhH7xpk-EpzaXr62beBz4GfjpCQh1T7xi~Q3sOGmWQnXfN87PZBnQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Figure 5.
Induction of the PC transcriptional program is intact in IL-21–stimulated STAT3MUT memory B cells. (A and B) Naive (CD20+CD10−CD27−IgG−; A) and memory (CD20+CD10−CD27+; B) B cells were sort-purified from normal donor controls (Ctl; n = 10 [or 7 for AICDA]), STAT3MUT patients (n = 5), or STAT1MUT patients (n = 4) and then cultured with CD40L alone or together with IL-21 (+IL-21) for 5 d. Expression of PAX5, PRDM1, XBP1, IRF4, and AICDA was determined by qPCR. The columns represent the mean ± SEM of experiments performed using naive B cells from 7–10 normal donors, 5 STAT3MUT patients, or 4 STAT1MUT patients. Levels of expression are relative to the amount of GAPDH. ns, not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001.
