CD38⁺ GL7⁺ cells are distinct from GC cells. (A) Representative flow cytometry analysis of CCR6, FAS, PNA, and Bcl-6 expression on PE-specific naive (gray) or CD38⁺ GL7⁺ (solid line) or CD38⁻ GL7⁺ (dashed line) GC cells, 6 or 7 d after injection. Each marker was analyzed in at least three animals. (B) Representative flow cytometry analysis of CD38 and GL7 expression by PE-specific B cells in PE-enriched samples from mixed bone marrow chimeras containing CD45.1⁺ WT and Bcl6^−/− cells at the noted times after injection. (C) Combined data from three experiments showing the total number of naive PE-specific WT and Bcl6^−/− B cells in uninjected chimeras and CD38⁺ GL7⁺ B cells in chimeras injected with PE/CFA. Numbers are corrected for chimerism based on the ratio of WT and Bcl6^−/− B cells that did not bind PE. The bars represent the means.