H. hepaticus–induced innate immune driven typhlocolitis is associated with IL-1β secretion. 129SvEv Rag2^−/− mice were infected with H. hepaticus and sacrificed >8 wk after infection. (A) IL-1β secretion from organ explants from colon, cecum, and ileum incubated overnight in complete medium. Results are shown as mean ± SEM (n = 3 for uninfected control and n = 14 for H. hepaticus–infected mice, pooled from 2 independent experiments). (B) IL-1β levels in the supernatants of cLPLs cultured overnight in complete media. Results are shown as mean ± SEM (n = 7 for uninfected controls and n = 22 for H. hepaticus-infected mice, pooled from 3 separate experiments). **, P < 0.01.