Heat map showing neutralization activity of mAbs and total IgG isolated from patient 3B. The IC₅₀ values for 3BC176, 3BC315, and total IgG on a panel of 39 viral strains comprising multiple clades compared with published data on the neutralization of the same viruses by 3BNC117 and 3BNC55 (Scheid et al., 2011). Top group displays viruses neutralized by both sets of antibodies (clone 1 and clone 2). Second and third group lists viruses that are neutralized only by 3BNC117 and 3BNC55 or only by 3BC176 and 3BC315, respectively. Three virus strains (bottom) were not neutralized by any of these antibodies. IC₅₀ values are color-coded: IC₅₀ < 0.1, red; IC₅₀ between 0.1 and 1 µg/ml, dark orange; IC₅₀ between 1 and 10 µg/ml, light orange; IC₅₀ between 10 and 100 µg/ml, dark yellow; and IC₅₀ > 100 µg/ml, yellow. IC₅₀ values above the measured concentration, as well as viruses that were not determined (nd), are not highlighted. All neutralization assays were performed in duplicate. Additionally, 3BC176 and 3BC315 were reproduced and retested against a panel of 10 different virus strains, confirming the neutralizing activity.