Airway DCs are motile but send few processes through the epithelium. (A) Standard motility of airway-adjacent DCs. Entire field for orientation (left) followed by zoomed images from relevant regions collected at the indicated times. (B) Sentinel-like motility of CD11c-EYFP cells on the inner surface of an airway obtained using two-photon microscopy and rendered in Imaris. (A and B) Yellow and red lines indicate tracks of two airway-adjacent DCs over a 30-min time lapse. (C) Process-forming DCs marked by CD11c-EYFP in Actin-CFP background. Process to probe epithelium is indicated by an arrow. Bars: (A, left) 25 µm; (A and C, right) 15 µm; (B and C, left) 50 µm; (B, right) 10 µm. Time stamps are shown in minutes/seconds. (D) Quantification of processes from airway DCs across airway epithelium in PBS- or OVA-challenged mice. (E) Track speed means of airway DCs from PBS- or OVA-challenged mice, before LPS treatment or after 10 min or 3 h of LPS treatment. Each dot represents one cell. Error bars represent SEM. Images are representative images from >12 mice per group. Quantification of track speed means are combined data from three separate experiments.