Figure 2.

Airway and alveolar DCs exhibit different behaviors. (A) Detailed image with overlaid tracks of cell motility at airway surfaces and alveolar spaces over the course of 2 h. The top half of the image is mostly alveoli, whereas the bottom is mostly airway. Arrows indicate alveolar DCs. Bar, 100 µm. (B) Track speed means calculated using Imaris for CD11c-EYFP DCs and AMs separated by airway and alveolar location in PBS and OVA challenge conditions. Each dot represents one cell. Error bars represent SEM. (C) Mean squared displacements ± SE as a function of time for alveolar or airway-adjacent DCs from untreated mice. (D) 30-min displacement for alveolar DCs from OVA-challenged mice with a 5-µm cutoff, which is the diameter of one cell. Each dot represents one cell. Data are from at least three independent experiments.

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