Figure 4. TCR diversity under conditions of thymus autonomy. TCR α and β chain analysis was performed on cDNA of total thymocytes derived from control mice (T; n = 2) or from grafts (G; n = 4). (A and B) Frequencies of V-J pairings are represented as heat maps for TCR β (A) and TCR α (B) chains. Each graph displays the number of times (in percentage; color in the bottom of panel B) defined V and J segments were paired in the TCR rearrangements identified. (C and D) The quantitative contribution of individual CDR3 clonotypes is represented as retrieval frequency (percentage of all TCR sequences) for β (C) and for α (D) chains, and was determined by counting identical CDR3 nucleotide sequences. The 10 most frequent CDR3 clonotypes are shown as colored bars, gray bars represent the remaining TCR sequences. Grafts were analyzed 10 wk after transplantation.
Figure 4.

TCR diversity under conditions of thymus autonomy. TCR α and β chain analysis was performed on cDNA of total thymocytes derived from control mice (T; n = 2) or from grafts (G; n = 4). (A and B) Frequencies of V-J pairings are represented as heat maps for TCR β (A) and TCR α (B) chains. Each graph displays the number of times (in percentage; color in the bottom of panel B) defined V and J segments were paired in the TCR rearrangements identified. (C and D) The quantitative contribution of individual CDR3 clonotypes is represented as retrieval frequency (percentage of all TCR sequences) for β (C) and for α (D) chains, and was determined by counting identical CDR3 nucleotide sequences. The 10 most frequent CDR3 clonotypes are shown as colored bars, gray bars represent the remaining TCR sequences. Grafts were analyzed 10 wk after transplantation.

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