TFF2 is required for inflammatory DCs to express IL-33 within the lung draining LNs after N. brasiliensis infection. (A) Gating strategy for the identification of inflammatory DCs as CD11b⁺, CD11c⁺, FcεR1⁺, and MHC class II⁺ within the MLNs. (B) Plots are gated on inflammatory DCs in MLNs of naive WT and TFF2^−/− mice or day 3 after infection with N. brasiliensis. Numbers indicate the percentage in each quadrant. (C) Percentage of IL-33⁺, TI/ST2⁺ inflammatory DCs from the experiment described in B. TI/ST2 (also known as IL-1RL1) is a critical subunit of the murine IL-33 receptor. Means ± SE of four mice/group are shown. Data are representative of three independent experiments (** P < 0.01).