TNF and IL-1β stimulate shape change of pericyte-like C3H/10T1/2 cells in vitro. (A) Representative fluorescence time-lapse microscopy images of Lifeact-EGFP transfected C3H/10T1/2 cells are shown for the indicated time points after cytokine (2 and 4 h) or vehicle control (PBS, unstimulated group). The 0 h time point was captured before cytokine or PBS addition. Bar, 100 µm. (B) Graphs show the percentage change in cell elongation over time in response to different concentrations of TNF (left) or IL-1β (right). Data were obtained after analysis of a minimum of seven independent experiments. Statistical significance between each time point after stimulation and the unstimulated condition (0 h time point) are indicated by asterisks. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Statistical significances between cytokine-stimulated (4 h) conditions and PBS-treated groups are indicated by hash symbols. #, P < 0.05; ##, P < 0.01. Images and results are from n = 5 experiments.