CD4⁺ T_N deficiency does not compromise CD4⁺ T_M homeostasis in progressive and partially suppressed SIV infection. (A–D) Comparison of the dynamics (absolute counts and proliferative fraction; mean + SEM) of overall CD4⁺ T_M and the T_CM, T_TrEM, and T_EM subsets in blood, the percentage of CD4⁺ of total CD3⁺ (memory) T cells in BAL, and absolute counts of CD4⁺ T_M with a regulatory phenotype (CD25⁺/FoxP3⁺) in blood of CD4⁺ T_N-depleted (red, n = 9) versus -repleted RMs (blue, n = 9) over the course of SIV infection (as in Fig. 1 A). The significance of differences in these parameters between RM groups in the indicated regions was assessed as described in the Materials and methods (significant p-values shown; NS, nonsignificant). (E) Comparison of the change in the percentage of Ki-67⁺ CD4⁺ T_M in peripheral LN of CD4⁺ T_N-depleted versus –repleted RMs between days 70 (ART day 0) and 73 (3 d after ART). Note that the increase in the percentage of Ki-67⁺ cells from day 70 to 73 was significant in both groups (p-values shown), but the magnitude of this increase (Δ%Ki-67) was not different between groups. (F and G) Comparison of the dynamics of CD8⁺ T_M in the blood of CD4⁺ T_N-depleted versus –repleted RMs over the course of SIV infection (mean + SEM), with statistical analysis of the indicated regions.