CD4⁺ T_N-deficient RMs display SIV replication dynamics similar to control RMs despite attenuated SIV-specific T cell and Ab responses. (A) Comparison of pvl, CD4⁺ T_M depletion, CD4⁺ T_M proliferative responses, and the magnitude of SIV-specific CD8⁺ T cell responses at day 70 pi between the CD4⁺ T_N -repleted control group (n = 9) and unmanipulated RMs (n = 6) challenged with the same SIVmac239 virus. Shown are box plots, with boxes and whiskers indicating interquartile ranges (25^th–75^th percentiles) and 10^th–90^th percentile values, respectively. Significance of differences in these parameters between groups was assessed as described in the Materials and methods (NS, nonsignificant). (B–G) Comparison of the indicated parameters (mean + SEM) over the course of SIVmac239 infection between the CD4⁺ T_N-depleted and CD4⁺ T_N-repleted groups (n = 9 each). The period over which all RMs received ART (day 70–156 pi) is shown in gray. The inset in E shows the percentage of RMs in the CD4⁺ T_N-depleted (red) and T_N-repleted (blue) RM groups that displayed a measureable anti-SIVenv neutralizing Ab response (e.g., seroconverted) before ART. Differences in these parameters between the CD4⁺ T_N-depleted and CD4⁺ T_N-repleted groups were statistically analyzed as described in the Materials and methods, with brackets indicating time points that were grouped together for analysis (significant p-values shown).