Figure 7.
Figure 7. sCD89 induces TGase2 expression on mesangial cells. (A) TGase2 immunostaining of frozen kidney sections from TGase2−/−, WT, α1KI, and α1KI-CD89Tg mice using a polyclonal anti-TGase2 antibody. (B) Detection of TGase2 expression by immunohistochemical analysis in kidney biopsies from a representative IgAN patient and a control with unrelated disease. (C) Immunostaining for TGase2 in frozen kidney sections of 6-wk-old α1KI mice injected i.v. with BSA or sCD89 (200 µg/mouse). Bars: (A) 10 µm; (B) 20 µm; (C) 5 µm. (D) Flow cytometry analysis of TGase2 expression on HMCs stimulated with IgA1 (gray layer) or sCD89 (white layer). The dotted layer corresponds to the isotypic-matched control. The histogram corresponds to one representative experiment of three.

sCD89 induces TGase2 expression on mesangial cells. (A) TGase2 immunostaining of frozen kidney sections from TGase2−/−, WT, α1KI, and α1KI-CD89Tg mice using a polyclonal anti-TGase2 antibody. (B) Detection of TGase2 expression by immunohistochemical analysis in kidney biopsies from a representative IgAN patient and a control with unrelated disease. (C) Immunostaining for TGase2 in frozen kidney sections of 6-wk-old α1KI mice injected i.v. with BSA or sCD89 (200 µg/mouse). Bars: (A) 10 µm; (B) 20 µm; (C) 5 µm. (D) Flow cytometry analysis of TGase2 expression on HMCs stimulated with IgA1 (gray layer) or sCD89 (white layer). The dotted layer corresponds to the isotypic-matched control. The histogram corresponds to one representative experiment of three.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal