Figure 4. sCD89 mediates mesangial IgA1 deposition in mice and is detected in glomeruli of patients. (A) Immunohistochemistry for the detection of CD89 deposits using the rabbit polyclonal anti-sCD89 in α1KI-CD89Tg versus α1KI and WT mice (12 wk old). (B) Detection of CD89 deposits by immunohistochemistry in biopsies of IgAN patients and controls using the rabbit polyclonal antibody pretreated or not with 500 µg/ml sCD89. Arrows indicate sCD89 deposits. (C) Immunostaining for human IgA in frozen kidney sections of 6-wk-old α1KI mice injected i.v. with BSA or sCD89. Bars: (A and C) 10 µm; (B) 20 µm. (D) Detection of IgA1–sCD89 complexes in kidney eluates from α1KI and α1KI-CD89Tg mice by ELISA using a monoclonal anti-CD89 (A3) and a polyclonal anti–human IgA antibody. Error bars indicate SEM. n = 7 mice per group. (E) Total IgA detected by ELISA in HPLC fractions of kidney eluates from α1KI-CD89Tg mice.
Figure 4.

sCD89 mediates mesangial IgA1 deposition in mice and is detected in glomeruli of patients. (A) Immunohistochemistry for the detection of CD89 deposits using the rabbit polyclonal anti-sCD89 in α1KI-CD89Tg versus α1KI and WT mice (12 wk old). (B) Detection of CD89 deposits by immunohistochemistry in biopsies of IgAN patients and controls using the rabbit polyclonal antibody pretreated or not with 500 µg/ml sCD89. Arrows indicate sCD89 deposits. (C) Immunostaining for human IgA in frozen kidney sections of 6-wk-old α1KI mice injected i.v. with BSA or sCD89. Bars: (A and C) 10 µm; (B) 20 µm. (D) Detection of IgA1–sCD89 complexes in kidney eluates from α1KI and α1KI-CD89Tg mice by ELISA using a monoclonal anti-CD89 (A3) and a polyclonal anti–human IgA antibody. Error bars indicate SEM. n = 7 mice per group. (E) Total IgA detected by ELISA in HPLC fractions of kidney eluates from α1KI-CD89Tg mice.

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