Figure 1. CD89 determines mesangial deposition of IgA1. (A) From left to right: immunostaining for human IgA1 in frozen kidney sections, double immunofluorescence for human IgA1 and CD31, PAS staining of paraffin-embedded kidney sections, and immunofluorescent staining for fibronectin in frozen sections from α1KI-CD89Tg versus α1KI and WT mice (12 wk old). Arrows indicate colocalization of IgA1 and CD31. (B) Transmission electron microscopy pictomicrographs of glomeruli from α1KI-CD89Tg versus α1KI and WT mice (12 wk old). C, capillary; E, endothelial cell; M, mesangial cell; P, podocyte. Arrows indicate dense materials. (C) Immunostaining for human IgA1 in frozen kidney sections of 6- and 24-wk-old α1KI-CD89Tg mice. Bars: (A and C) 10 µm; (B) 2 µm. (D) Dose-dependent interaction of mouse IgA with sCD89 monitored by real-time surface plasmon resonance (SPR).(E) Association of different types of IgA (mouse IgA, α1KI mouse IgA1, and human IgA1; 5 µM) with sCD89.
Figure 1.

CD89 determines mesangial deposition of IgA1. (A) From left to right: immunostaining for human IgA1 in frozen kidney sections, double immunofluorescence for human IgA1 and CD31, PAS staining of paraffin-embedded kidney sections, and immunofluorescent staining for fibronectin in frozen sections from α1KI-CD89Tg versus α1KI and WT mice (12 wk old). Arrows indicate colocalization of IgA1 and CD31. (B) Transmission electron microscopy pictomicrographs of glomeruli from α1KI-CD89Tg versus α1KI and WT mice (12 wk old). C, capillary; E, endothelial cell; M, mesangial cell; P, podocyte. Arrows indicate dense materials. (C) Immunostaining for human IgA1 in frozen kidney sections of 6- and 24-wk-old α1KI-CD89Tg mice. Bars: (A and C) 10 µm; (B) 2 µm. (D) Dose-dependent interaction of mouse IgA with sCD89 monitored by real-time surface plasmon resonance (SPR).(E) Association of different types of IgA (mouse IgA, α1KI mouse IgA1, and human IgA1; 5 µM) with sCD89.

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