HIF1 regulation of chemokines and chemokine receptors. (A) A comparison of the transcriptional profile of WT versus HIF1^−/− CTLs was performed by microarray. Shown here are KEGG pathway analysis of genes up-regulated in HIF1^−/− CTLs (top) and a heat map showing the relative normalized expression of selected genes that are significantly different in expression in WT versus HIF1^−/− CTLs, as determined by microarray. (B) Real-time PCR analysis of CD62L expression in WT and HIF1^−/− CTLs. (C) Analysis of CD62L surface expression on WT and HIF1^−/− CTLs by flow cytometry. (D) Real-time PCR analysis of CCR7 expression in WT and HIF1^−/− CTLs. (E) WT and HIF1^−/− CTLs were labeled with CFSE or CellTracker orange (CMTMR) and mixed at a ratio of 1:1 before being injected into C57BL/6 host mice. Values indicate recovery of WT or HIF1^−/− cells as a percentage of the total recovered transferred cells from the blood and lymph nodes 4 h after transfer. Each dot indicates a mouse; horizontal bars indicate mean. (F) P14 T cells were activated for 2 d with cognate peptide and then cultured for a further 4 d with IL-2 in different glucose concentrations. Cells were then analyzed for the surface expression of CD62L by flow cytometry. In B and D, mean ± SEM of three experiments performed in triplicate is shown; in C and F, data are representative of at least three experiments (*, P < 0.05; **, P < 0.01).