Figure 4. NK cells and neutrophils are localized in the same areas of the spleen and the lymph nodes and form conjugates. (A) Immunostaining on NKp46iCre/wtR26ReYFP/wt spleen (top) or lymph node (bottom) sections at steady state showing neutrophils (anti-MMP9, red), NK cells (anti-GFP, green), B cells (anti-B220, blue), and the lymphatic vessels (Lyve-1, white in the lymph node image). Adjacent overlapping confocal images of spleen and lymph node sections were stitched to generate panoramic images. White arrowheads show contact between neutrophils and NK cells in the spleen. (B) Percentages of conjugates formed between neutrophils and NK cells (NK cells + Neutro), CD3+ T cells (T cells + Neutro), or CD3− NK1.1− cells (B cells + Neutro) after 10 min incubation together in vitro at 37°C. Data are representative of three independent experiments. Statistical significance was determined with a Mann-Whitney test. *, P < 0.05; **, P < 0.01; ***, P < 0.001.
Figure 4.

NK cells and neutrophils are localized in the same areas of the spleen and the lymph nodes and form conjugates. (A) Immunostaining on NKp46iCre/wtR26ReYFP/wt spleen (top) or lymph node (bottom) sections at steady state showing neutrophils (anti-MMP9, red), NK cells (anti-GFP, green), B cells (anti-B220, blue), and the lymphatic vessels (Lyve-1, white in the lymph node image). Adjacent overlapping confocal images of spleen and lymph node sections were stitched to generate panoramic images. White arrowheads show contact between neutrophils and NK cells in the spleen. (B) Percentages of conjugates formed between neutrophils and NK cells (NK cells + Neutro), CD3+ T cells (T cells + Neutro), or CD3− NK1.1− cells (B cells + Neutro) after 10 min incubation together in vitro at 37°C. Data are representative of three independent experiments. Statistical significance was determined with a Mann-Whitney test. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

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